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| 产品名称： | LB5871-LYMP |
|---|---|
| 商品货号： | TS260482 |
| <strong>Cell line </strong>： | LB5871-LYMP |
| DSMZ no. ： | TS260482 |
| Species ： | human |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 2016 from a cervical lymph node of a 64-year-old woman with diffuse large B-cell lymphoma (GCB type, stage IIIA) that was transformed from an earlier diagnosed follicular lymphoma. Patient also had a history of breast cancer. Cells are described to exhibit a complex karyotype including BCL2 and MYC translocations (double-hit DLBCL). |
| Reference(s) ： | 18202 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells growing in suspension; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at 0.7 x 106 cells/ml in a 24-well-plate; maintain at 0.5-1.4 x 106 cells/ml, split 1:2 every 3 days; we recommend to cultivate the cells in 24-well-plates continuously |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70 hours |
| Harvest ： | maximal density of ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, sm/cylambda - ; image |
| Fingerprint ： | standardized STR9 (ANSI/ASN-0002-2011) revealed a unique STR DNA profile that matches with the donor |
| Molec. Genetics ： | expression of BCL2 and MYC confirmed by western blotting |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, XMLV - |