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| 产品名称： | UPCI-SCC-036 |
|---|---|
| 商品货号： | TS260487 |
| <strong>Cell line </strong>： | UPCI-SCC-036 |
| DSMZ no. ： | TS260487 |
| Species ： | human |
| Cell type ： | squamous cell carcinoma |
| Origin ： | established in 1993 from a new, poorly differentiated primary tumor of the tonsils (grade 2,T3N1) of a 56-year-old man with head and neck squamous cell carcinoma. Described to carry a chromosomal 11q13 amplification and several other aberrations. The cell line will only be distributed to non-profit institutions, commercial entities may apply for a license from Maria Vanegas (msv4@pitt.edu / 001-414-648-4004) at the University of Pittsburgh, Office of Technology Management |
| Reference(s) ： | 15061, 14735, 15176 |
| Biosafety level ： | 1 |
| Risk assessment ： | Originally, cells were described to be HPV-11 positive but the accessioned cell line was tested as HPV negative |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently as monolayer; image; image |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS + non-essential amino acids + 2 mM L-glutamine |
| Subculture ： | seed out at about 1 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 once or twice a week using trypsin/EDTA (incubate up to 10 min with trypsin) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | cell harvest of ca. 3-5 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Plasmocin, then negative in PCR asay |
| Immunology ： | to make inquiries about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | standardized STR9 (ANSI/ASN-0002-2011) revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, XMLV - |