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| 产品名称： | OVPA-8 |
|---|---|
| 商品货号： | TS260493 |
| <strong>Cell line </strong>： | OVPA-8 |
| DSMZ no. ： | TS260493 |
| Species ： | human |
| Cell type ： | ovarian cancer |
| Origin ： | established in 2011 from the ascites fluid of a 47-year-old woman with recurrent high-grade serous ovarian cancer after three different chemotherapies (diagnosed in 2005 with stage III C papillary serous adenocarcinoma G3). Cells are described to exhibit multiple chromosomal aberrations, TP53 and BRCA1 mutations and the loss of one copy of BRCA2. Also described to be resistant to cisplatin and AZD4547, but sensitive to paclitaxel and CPL304-110-01 |
| Reference(s) ： | 18203 |
| Biosafety level ： | 1 |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently as monolayer, carrying many intracellular dark granules; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at about 1 x 106 cells/80 cm2; split subconfluent (70-80%) culture 1:5 - 1:10 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | 50-60 hours |
| Harvest ： | cell harvest of ca. 10 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Fingerprint ： | standardized STR9 (ANSI/ASN-0002-2011) revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, XMLV - |