微信小陈
微信小章
产品简介
购买须知
| 产品名称： | Clostridium difficile |
|---|---|
| 商品货号： | TS274027 |
| 中文名称： | 艰难梭菌 |
| 模式菌株： | 模式菌株 |
| 其它保藏中心编号： | ATCC9689 |
| 特征特性： | Nucleotide (GenBank) : X94328 C.difficile spo0A gene. Nucleotide (GenBank) : AX110003 Sequence 736 from Patent WO0123604. Nucleotide (GenBank) : AX110007 Sequence 740 from Patent WO0123604. Nucleotide (GenBank) : AX110095 Sequence 828 from Patent WO0123604. Nucleotide (GenBank) : AX111484 Sequence 2217 from Patent WO0123604. Nucleotide (GenBank) : AX111505 Sequence 2238 from Patent WO0123604. Nucleotide (GenBank) : AX111525 Sequence 2258 from Patent WO0123604. Nucleotide (GenBank) : U36481 Clostridium difficile sporulation protein homolog (spo0A) gene, partial cds. Nucleotide (GenBank) : S51968 {16S/23S ribosomal DNA spacer region} Clostridium difficile, ATCC 9689, Genomic, 271 nt Nucleotide (GenBank) : U43514 Clostridium difficile putative spoIVB (spoIVB) gene, complete cds, and phosphorylation-activated transcription factor spo0A (spo0a) gene, complete cds. Rolfe RD, Finegold SM. Purification and characterization of Clostridium difficile toxin. Infect. Immun. 25: 191-201, 1979. PubMed: 478634 Florin I, Thelestam M. Intoxication of cultured human lung fibroblasts with Clostridium difficile toxin. Infect. Immun. 33: 67-74, 1981. PubMed: 6167521 Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980. Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999 Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986 Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303 Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999 Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980. Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999 |
| 具体用途： | 科研，生产，产生细胞毒素,肠内研究 |
| 培养基： | ATCC® Medium 2107: Modified Reinforced Clostridial ATCC® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood |
| 培养温度： | 37 ℃, 厌氧气体混合物,80% N2 -10% CO2 -10% H2 |
| 冻干物说明： | ·冻干首次活化,干粉要全部用完,不能保留,用0.2-0.5m1的培养液或者无菌水溶解,接种在1-2个平板上,因冻干菌种处于休眠状态,请勿接种多个平板,以免因接种量不足而导致复苏不成功； |
| 保存说明： | 1、长期保存的储存菌株以冷冻干燥保藏为主，特殊要求的菌种可用其它方法进行保存，保存期可长达几年至几十年。2、半储存菌株以含20%甘油的肉汤或液体石腊封存的半固体琼脂为主，在0℃以下保存，保存期为3－6个月。3、应用菌株以琼脂斜面为主，在4℃保存，保存期为1－3个月。4、菌种的传代不可超过6次。视菌种的特性及使用情况，一般储存菌株每5年传记代一次，每1-2年从储存菌株转接至半储存菌株，半储存菌株每3-6个月传代一次，每1-3个月从半储存菌株转接接至应用菌株。 |