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| NCIMB number | NCIMB 11935 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | No |
| Taxon name | Lysinibacillus sphaericus |
| Depositor designation | N.R.Smith966 Claussen1911 |
| Preservation method | Lyophilised |
| Preserved as | Bacillus sphaericus |
| Price band | A |
| Media | 001 |
| Gas regime | aerobic |
| Growth factors (and/or information) | CM3, 25C, 3d: good growth, soft easily dispersed. At 37C occasional opaque (sporing) colony. |
| ACDP category | 1 |
| Colony Edge | Entire |
| Colony Surface | Smooth/Shiny |
| Colony Shape | Circular |
| Colony Elevation | Slightly Raised |
| Colony Colour | Off White |
| Colony Opacity | Translucent |
| Cellular Shape | Rod |
| Cellular Motility | Yes |
| Gram stain | Gram Positive |
| Cell inclusion bodies | Spores - Terminal, Distending, Spherical |
| Depositor Company | American Type Culture Collection (ATCC) |
| Depositor Address | 12301 Parklawn Drive Rockville Maryland 20852 U.S.A. |
| Date of Accession | 02/12/1983 |
| History | N.R.Smith -- J.R.Porter -- N.H.Claussen |
| Other collection IDs | ATCC10208 CCM1087 DSM5019 IFO3525 |
| Yeast? | False |
| K12 | No |
| References | Smith N.E., Gordon R.E. and Clark F.E. (1946) USDA Miscellaneous Publications 559. Aerobic Mesophilic Sporeforming Bacteria. Smith N.R., Gordon R.E. and Clark F.E. (1952) Agricultural Monograph no 16 USDA. Aerobic Sporeforming Bacteria. Izumi et al (1972) Agric. biol. Chem. 36 p510 Izumi et al (1973) Agric. biol. Chem. 37 p1327 Izumi et al (1973) Agric. biol. Chem. 37 p1335 Izumi Y., Tani Y. and Ogata K. (1979) Meth. Enzym. 62D pp326-338. Microbiological synthesis of biotin. Izumi Y. et al (1981) Agric. biol. Chem. 45 pp1983-1989. Characterisation of biotin biosynthetic enzymes of Bacillus sphaericus: a dethiobiotin producing bacterium. Azoulay M., Frappier F. and Marquet A. (1985) Agric. biol. Chem. 49 pp2783-2785. On the biosynthesis of biotin: bioconversion of 1,3-dimethyldethiobiotin by Bacillus sphaericus. Int J Syst Evol Microbiol (2007) 57, 1117-1125 Aerobic spore forming bacteria, US Dep Agric Monogr 16, 1-148, 1952 Aerobic mesophilic spore-forming bacteria, US Dep Agric Misc Publ 559, 41, 1946 Cloning of the biotin synthetase gene from Bacillus sphaericus and expression in Escherichia coli and Bacilli, Gene 80(1), 39-48, 1989 |