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| NCIMB number | NCIMB 8919 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | No |
| Taxon name | Geobacillus stearothermophilus |
| Depositor designation | N.R.SmithT73 |
| Preservation method | Lyophilised |
| Other catalogue information | Used in the preparation of spore papers for testing of steam sterilizers |
| Price band | A |
| Media | 090 |
| Gas regime | aerobic |
| Growth factors (and/or information) | strain 1356: B12, guanine, cytidine; strain 73: methionine; NaCl tolerance 5%-55% CM55, pH7.0, 55C, 20hr: good growth, consistency butyrous, 2 colony types - 1 light, 1dark, dark one has more spores |
| ACDP category | 1 |
| Colony Edge | Entire |
| Colony Surface | Smooth |
| Colony Shape | Circular |
| Colony Elevation | Flat |
| Colony Colour | White/Off White |
| Colony Opacity | Opaque |
| Cellular Shape | Group Ii Rod |
| Cellular Size | medium but short |
| Cellular Arrangement | Singles, Groups |
| Cellular Motility | Yes |
| Gram stain | Gram Variable |
| Cell inclusion bodies | Spores - Terminal, Sporangium Swollen |
| Cellular (Other) | Straight Axis, Parallel Sides, Rounded Ends |
| Depositor Company | American Type Culture Collection (ATCC) |
| Depositor Address | 12301 Parklawn Drive Rockville Maryland 20852 U.S.A. |
| Source | Deteriorated canned food |
| Date of Accession | 01/11/1957 |
| Other collection IDs | ATCC12976 CCEB666 CCM2183 DSM297 HNCMB102003 IFO12983 IMET10307 NCA1356 VKMB-719 NCDO2011 |
| Yeast? | False |
| K12 | No |
| References | Baker H. et al (1960) Can. J. Microbiol. 6 p557 Kelsey J.C. (1961) J. clin. Path. 14 p313. The testing of sterilisers. 2 Thermophilic spore papers. Cook A.M. and Brown M.R.W. (1964) J. Pharm. Pharmac. 16 pp725-732. The relation between heat activation and colony formation for the spores of Bacillus stearothermophilus. Cook A.M. and Gilbert R.J. (1968) J. Pharm. Pharmac. 20 p626. Effect of storage conditions on the heat resistance and heat activation response of B. stearothermophilus spores. Brown M.R.W. and Melling J. (1973) J. Pharm. Pharmac. 25 p478. Release of dipicolinic acid and calcium and activation of Bacillus stearothermophilus spores as a function of time, temperature and pH. Wright A.M. et al (1995) J. appl. Bact. 79 pp432-438 Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. th, Int J Syst Evol Microbiol 51(2), 433-446, 2001 Growth requirements of 94 strains of thermophilic bacilli, Can J Microbiol 6, 557-563, 1960 The examination of spoiled canned foods. 2. Classification of flat sour, spoilage organisms from non acid foods, J Infect Dis 39, 89-105, 1962 |