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| NCIMB number | NCIMB 503 |
| Deposit type | Bacteria |
| Type strain | Yes |
| GMO | No |
| Taxon name | Clostridium histolyticum |
| Depositor designation | Weinberg |
| Preservation method | Lyophilised |
| Price band | A |
| Media | 012 |
| Gas regime | anaerobic |
| Growth factors (and/or information) | Heated blood agar, 37C, 24hr: good growth, butyrous consistency, variation in opacity with numbers of spores in colony. |
| ACDP category | 2 |
| Hazard information | This species has been associated with gas gangrene and war wounds. Pathogenic to laboratory animals. Has been isolated from human gut, gingival plaque and soil. |
| Colony Surface | Smooth |
| Colony Shape | Irregular/Circular |
| Cellular Shape | Rod |
| Cellular Arrangement | Singly/Pairs |
| Cellular Motility | Yes |
| Gram stain | Gram Positive |
| Cell inclusion bodies | Spores - Oval, Sub Terminal |
| Cellular (Other) | Rounded Ends |
| Depositor Company | National Collection of Type Cultures (NCTC) |
| Depositor Address | 61 Colindale Avenue London U.K. NW9 5HT |
| Source | Gas gangrene |
| Isolated by | Weinberg |
| Date of Isolation | 01/01/1916 |
| Date of Accession | 13/12/1951 |
| History | M.Robertson -- M.Weinberg |
| Other collection IDs | ATCC19401 DSM2158 JCM1403 NCTC503 |
| Yeast? | False |
| K12 | No |
| References | Weinberg M. and Seguin P. (1916) c.R. Acad. Sci. 163 p449 Weinberg M. and Seguin P. (1917) c.R. Soc. Biol. 80 p157 Hobbs G. (1958) Thesis. Edwards D.J. et al (1973) J. gen. Microbiol. 76 p135 (Unknown), Int J Syst Bacteriol 30, 280, 1980 Approved Lists of Bacterial Names, Int J Syst Bacteriol 30, 225-420, 1980 The amino acid-fermenting clostridia, J Gen Microbiol 67, 47-56, 1971 Amino acid utilization patterns in clostridial taxonomy, Arch Microbiol 123, 137-141, 1979 Cell wall composition and deoxyribonucleic acid similarities among the anaerobic coryneforms, classical propionibacteria, and strains of Arachnia propionica, J Bacteriol 109(3), 1047-1066, 1972 Cloning and nucleotide sequence analysis of the colH gene from Clostridium histolyticum encoding a collagenase and a gelatinase, J Bacteriol 176(21), 6489-6496, 1994 Electrophoretic study of Clostridium species, J Clin Microbiol 15(4), 688-702, 1982 Gene duplication and multiplicity of collagenases in Clostridium histolyticum, J Bacteriol 181(3), 923-933, 1999 The end products of the metabolism of aromatic amino acids by clostridia, Arch Microbiol 107, 283-288, 1976 The lipid fatty acids of proteolytic clostridia, J Gen Microbiol 118, 115-123, 1980 US General Services Administration, Test kit, anaerobe identification Washington, DC, US General Services Administration; Commercial Item Description A-A-51238. |