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| NCIMB number | NCIMB 775 |
| Deposit type | Bacteria |
| Type strain | Yes |
| GMO | No |
| Taxon name | Enterococcus faecalis |
| Depositor designation | Tissier |
| Preservation method | Lyophilised |
| Preserved as | Streptococcus faecalis |
| Price band | A |
| Media | 079 |
| Gas regime | aerobic |
| Growth factors (and/or information) | Blood agar, 37C, 24hr: no haemolysis |
| ACDP category | 2 |
| Hazard information | This genus has been associated with nosocomial infections, especially of urinary tract. Associated with subacute endocarditis. |
| Additional hazard information | Ref: R C Moellering, Clinical Infectious Diseases 1992, 14, 1173-8. Emergence of Enterococcus as a significant pathogen. |
| Colony Edge | Entire |
| Colony Surface | Smooth |
| Colony Shape | Circular |
| Colony Colour | Colourless |
| Colony Opacity | Translucent |
| Cellular Shape | Coccus |
| Cellular Arrangement | Pairs/Short Chains |
| Cellular Motility | No |
| Gram stain | Gram Positive |
| Depositor Company | National Collection of Type Cultures (NCTC) |
| Depositor Address | 61 Colindale Avenue London U.K. NW9 5HT |
| Date of Accession | 13/12/1951 |
| History | C.L.Hannay |
| Other collection IDs | ATCC19433 CIP103015 DSM20478 NCDO581 NCTC775 |
| WDCM number | WDCM 00009 |
| Yeast? | False |
| K12 | No |
| References | Fuller R. and Newland L.G.M. (1963) J. gen. Microbiol. 31 pp431-434 Jones D. et al (1972) J. gen. Microbiol. 72 p439. A numerical taxonomic study of streptococci of serological group D. Andrew M.H.E. (1973) J. gen. Microbiol. 74 p195. Use of Trimethoprim to obtain thymine-requiring mutants of S. faecalis. Staples D.G. and Fry J.C. (1973) J. appl. Bact. 36 p1 Mead G.C. (1974) J. gen. Microbiol. 82 p421 J Appl Micro (1998) 85, 25-36 IJSEM (2002) 52:1122 IJSEM (2003) 53:718 The tmRNA website: reductive evolution of tmRNA in plastids and other endosymbionts, Nucleic Acids Res 32(Databaseissue), D104-D108, 2004 Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes, Microbiology 151(7), 2141-2150, 2005 cpnDB: a chaperonin sequence database, Genome Res 14(8), 1669-1675, 2004 Designation of the provisional new enterococcus species CDC PNS-E2 as Enterococcus sanguinicola sp. nov., isolated from human blood, and identification of a strain previously named Enterococcus CDC PNS-E1 as Enterococcus italicus Fortina, Ricci, Mora, and Manachini 2004, J Clin Microbiol 46(10), 3473-3476, 2008 Enterococci in Water by Membrane Filtration Using membrane-Enterococcus-Esculin Iron Agar.Washington, DC: Environmental Protection Agency, (journal unknown) , EPA EPA Method 1106.1, 2005 Enterococci in Water by Membrane Filtration Using membrane-Enterococcus Indoxyl-B-D-Glucoside Agar.Washington, DC: Environmental Protection Agency, (journal unknown) , EPA EPA Method 1600, 2005 Escherichia coli in Water by Membrane Filtration using membrane-Thermotolerant Escherichia coli Agar (mTEC).Washington, DC: Environmental Protection Agency, (journal unknown) , EPA EPA Method 1103.1, 2005 Escherichia coli in Water by Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar.Washington, DC: Environmental Protection Agency, (journal unknown) , EPA EPA Method 1603, 2005 Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermenation using Lauryl Tryptose Broth (LTB) and EC Medium.Washington, DC: Environmental Protection Agency, (journal unknown) , EPA EPA Method 1680, 2005 Identification of clinically relevant enterococcus species by direct sequencing of groES and spacer region, J Clin Microbiol 43(1), 235-241, 2005 Identification of Clinically Relevant Viridans Group Streptococci by Sequence Analysis of the 16S-23S Ribosomal DNA Spacer Region, J Clin Microbiol 42(6), 2651-2657, 2004 Identification of Enterococcus species and phenotypically similar Lactococcus and Vagococcus species by reverse checkerboard hybridization to chaperonin 60 gene sequences, J Clin Microbiol 38(11), 3953-3959, 2000 Identification of Enterococcus spp. based on specific hybridisation with 16S rDNA probes, J Microbiol Methods 50(2), 115-121, 2002 Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media..Geneva (Switzerland): International Organization for Standardization/ANSI, (journal unknown) , ISO ISO 11133-2, 2003 Molecular cloning, overexpression, purification, and characterization of an aerobic FMN-dependent azoreductase from Enterococcus faecalis, Protein Expr Purif 34(2), 302-310, 2004 Mycolic acids from Rhodococcus, Gordonia, and Dietzia, J Microbiol Methods 40(1), 1-9, 2000 Partial recN gene sequencing: a new tool for identification and phylogeny within the genus Streptococcus, Int J Syst Evol Microbiol 60(9), 2140-2148, 2010 Phylogeny and identification of Enterococci by atpA gene sequence analysis, J Clin Microbiol 43(5), 2224-2230, 2005 Reevaluation of the Taxonomic Status of Recently Described Species of Enterococcus: Evidence that E. thailandicus Is a Senior Subjective Synonym of "E. sanguinicola" and Confirmation of E. caccae as a Species Distinct from E. silesiacus, J Clin Microbiol 49(7), 2676-2679, 2011 rpoB Gene Sequence-Based Identification of Aerobic Gram-Positive Cocci of the Genera Streptococcus, Enterococcus, Gemella, Abiotrophia, and Granulicatella, J Clin Microbiol 42(2), 497-504, 2004 Sequencing the gene encoding manganese-dependent superoxide dismutase for rapid species identification of enterococci, J Clin Microbiol 38(1), 415-418, 2000 The rpoB gene as a target for PCR-DGGE analysis to follow lactic acid bacterial population dynamics during food fermentations, Food Microbiol 21(4), 481-487, 2004 Use of competitive DNA hybridization to identify differences in the genomes of bacteria, J Microbiol Methods 66(2), 321-330, 2006 Use of the manganese-dependent superoxide dismutase gene sodA for rapid identification of recently described enterococcal species, Folia Microbiol (Praha) 57(5), 439-442, 2012 Utilization of tmRNA sequences for bacterial identification, BMC Microbiol 1, 20, 2001 |