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| NCIMB number | NCIMB 9481 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | No |
| Taxon name | Escherichia coli |
| Depositor designation | Lederberg W1485 |
| Preservation method | Lyophilised |
| Other catalogue information | "A lambda- derivative. Classical F+ donor in conjugation. Carries an autonomous wild type sex factor. Non- lysogenic. Indicator strain lysed by phage lambda, carried by strain K12 wild type (ATCC 10798)" |
| Price band | A |
| Media | 001 |
| Gas regime | aerobic |
| Growth factors (and/or information) | L-L agar, 37C, 24hrs: metallic sheen, butyrous consistency |
| ACDP category | 1 |
| Hazard information | This species has been associated with diarrhoea, urinary tract infections. |
| Colony Edge | Entire |
| Colony Surface | Shiny/Rough |
| Colony Shape | Circular |
| Colony Elevation | Low Convex |
| Colony Colour | Grey-White |
| Colony Opacity | Opaque |
| Cellular Shape | Rod |
| Cellular Size | 0.5 x 2-3 u |
| Cellular Motility | Yes |
| Gram stain | Gram Negative |
| Cellular (Other) | Straight Axis, Parallel Sides, Rounded Ends |
| Depositor Name | W.Hayes |
| Depositor Company | Microbial Genetics Research Unit |
| Depositor Address | Hammersmith Hospital, Du Cane Road London U.K. W12 |
| Isolated by | J. Lederberg |
| Date of Accession | 15/10/1963 |
| Other collection IDs | ATCC12435 DSM5695 IFO3302 |
| Genotype information | "F+, met, str-r, T1-s, T6-s, lambda-" |
| Yeast? | False |
| K12 | Yes |
| References | EPO Patent Appl. P25 35 554 8 - 41 published 21/9/82. Furness G. and Rowley D. (1957) J. gen. Microbiol. 17 p550 Izaki K. and Arima K. (1963) Nature 200 p384 Paigen K. (1963) Biochim. biophys. Acta 77 p318 Yura T., Marushige K. and Imai M. (1963) Biochim. biophys. Acta 76 p442 Webb S.J. (1968) Nature 217 Sourcebook of Experiments for the Teaching of Microbiology, Eds. S.B. Primrose and A.C. Wardlaw, Academic Press 1982. [Expt. 49, 111] A novel real-time polymerase chain reaction-based method for the detection and quantification of lactose-fermenting Enterobacteriaceae in the dairy and other food industries, J Dairy Sci 93(3), 860-867, 2010 Filamentous coliphage M13 as a cloning vehicle in the M13 replicative form in vitro, Proc Natl Acad Sci USA 74, 3642-3646, 1977 Genetic Studies of Lysogenicity in Escherichia Coli, Genetics 38(1), 51-64, 1953 |