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| NCIMB number | NCIMB 11187 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | No |
| Taxon name | Escherichia coli |
| Depositor designation | WP2 |
| Preservation method | Lyophilised |
| Other catalogue information | Derived from strain B/r by UV irradiation and penicillin selection. Non pathogenic |
| Price band | A |
| Media | 001 |
| Gas regime | aerobic |
| Growth factors (and/or information) | CM3, 30C, 1d: good growth |
| ACDP category | 1 |
| Additional hazard information | Although presently classed as ACDP group 1, this organism is recognised as an opportunist pathogen, particularly of immunocompromised patients, and should be handled with care. |
| Colony Edge | Entire |
| Colony Surface | Smooth |
| Colony Shape | Circular |
| Colony Elevation | Flat |
| Colony Colour | Off White |
| Colony Opacity | Translucent |
| Cellular Shape | Rod |
| Cellular Size | small |
| Cellular Arrangement | Single, Pairs |
| Cellular Motility | No |
| Gram stain | Gram Negative |
| Cellular (Other) | Straight Axis, Parallel Sides, Rounded Ends |
| Depositor Name | M.H.L.Green |
| Depositor Company | MRC Cell Mutation Unit |
| Depositor Address | University of Sussex, Falmer Brighton Sussex U.K. |
| Isolated by | E.M. Witkin |
| Date of Isolation | 01/01/1956 |
| Date of Accession | 01/04/1975 |
| Other collection IDs | ATCC49980 DSM9494 |
| Genotype information | trp |
| Yeast? | False |
| K12 | No |
| References | Witkin E.M. (1956) Cold Springs Harbor Symp. Quant. Biol. 21 pp123-140 Hill R.F. (1965) Photochem. Photobiol. 4 p563 Bridges B.A. et al (1972) Chem- Biol. Interactions 5 pp77-84 Witkin E.M. and George D.L. (1975) Genetics Green M.H.L. and Muriel W.J. (1975) Mutation Res. Ashwood Smith M.J. and Grant E. (1976) Cryobiology 13 pp206-213 Brusick D.J. et al (1980) Mutation Res. 76 pp169-190. An evaluation of the E. coli WP2 and WP2 uvrA reverse mutation assay. An evaluation of the Escherichia coli WP2 and WP2 uvrA reverse mutation assay, Mutat Res 76(2), 169-190, 1980 Dose-mutation relationships in ultraviolet-induced reversion from auxotrophy in Escherichia coli, J Gen Microbiol 30, 281-287, 1963 Mutation induction in bacteria by freeze-drying, Cryobiology 13(2), 206-213, 1976 Repair-deficient bacterial strains suitable for mutagenicity screening: tests with the fungicide captain, Chem Biol Interact 5(2), 77-84, 1972 Use of repair-deficient strains of Escherichia coli and liver microsomes to detect and characterise DNA damage caused by pyrrolizidine alkaloids heliotrine and monocrotaline, Mutat Res 28(3), 331-336, 1975 |