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| NCIMB number | NCIMB 15116 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | Yes |
| Taxon name | Pseudomonas sp. |
| Depositor designation | DF57-Cu15 |
| Preservation method | Liquid nitrogen & Lyophilised |
| Preserved as | Pseudomonas sp. |
| Other catalogue information | Recombinant strain (GMO) with an inserted promoter-less Tn5:luxAB cassette controlled by a copper-inducible promoter in the DF57 host strain. As a result, the strain is bioluminescent. The inserted transposon also carries a gene conferring resistance to kanamycin. |
| Price band | A |
| Media | 001069112 |
| Gas regime | Aerobic |
| Incubation period | 1 day |
| Growth factors (and/or information) | Luria-Bertani (LB) agar with kanamycin (25 mg/L) for reactivation of cryo stocks. Davis Minimal Medium supplemented with kanamycin (25 mg/L) for routine growth in liquid medium. |
| ACDP category | 1 |
| Colony Edge | Entire |
| Colony Surface | Glossy |
| Colony Shape | Circular |
| Colony Elevation | Convex |
| Colony Colour | Yellow/Cream |
| Colony Opacity | Translucent |
| Cellular Shape | Rods |
| Cellular Size | Small |
| Cellular Arrangement | Singles |
| Gram stain | Gram negative |
| Cellular (Other) | Parallel sides, rounded ends |
| Depositor Name | Kristian Koefoed Brandt |
| Depositor Company | University of Copenhagen, Department of Plant and Environmental Sciences, Section for Microbial Ecology and Biotechnology |
| Depositor Address | Thorvaldsensvej 40, DK-1871 Frederiksberg, Denmark |
| Source | The host strain (DF57) was isolated from cucumber rhizosphere soil from Greenhouse in Taastrup near Copenhagen around 1990. |
| Isolated by | Dan Funch Jensen |
| Date of Isolation | 1990 |
| Date of Accession | 16/02/2018 |
| History | The host strain was stored (glycerol cryostocks) before being used to generate strain DF57-Cu15 by TN5::luxAB mutagenesis (Kragelund et at., 1995; Tom-Petersen et al., 2001). Since then, the strain has been maintained as glycerol cryostocks (15% vol/vol ). |
| References | Kragelund et al. (1995). Isolation of lux reporter gene fusions in Pseudomonas fluorescens DF57 inducible by nitrogen or phosphorus starvation. FEMS Microbiol Ecol 17:95-106. Tom-Petersen et al. (2001). Identification of copper-induced genes in Pseudomonas fluorescens and use of a reporter strain to monitor bioavailable copper in soil. FEMS Microbiol Ecol 38:59-67. |