KCl |
0.34 |
g |
MgCl2 x 6 H2O | 4.00 | g |
MgSO4 x 7 H2O | 3.45 | g |
NH4Cl | 0.25 | g |
CaCl2 x 2 H2O | 0.14 | g |
K2HPO4 | 0.14 | g |
NaCl | 18.00 | g |
Trace element solution (see below) | 10.00 | ml |
Fe(NH4)2(SO4)2 x 6 H2O solution (0.1% w/v) | 2.00 | ml |
Na-acetate | 1.00 | g |
Yeast extract (OXOID) | 2.00 | g |
Trypticase peptone (BD BBL) | 2.00 | g |
Na-resazurin solution (0.1% w/v) | 0.50 | ml |
NaHCO3 | 5.00 | g |
Vitamin solution (see below) | 10.00 | ml |
L-Cysteine-HCl x H2O | 0.50 | g |
Na2S x 9 H2O | 0.50 | g |
Distilled water | 1000.00 | ml |
Note: If the medium is being used without overpressure then adjust pH with a small amount of sterile anoxic 1 N HCl, if necessary.
Trace element solution: Nitrilotriacetic acid |
1.50 |
g |
MgSO4 x 7 H2O | 3.00 | g |
MnSO4 x H2O | 0.50 | g |
NaCl | 1.00 | g |
FeSO4 x 7 H2O | 0.10 | g |
CoSO4 x 7 H2O | 0.18 | g |
CaCl2 x 2 H2O | 0.10 | g |
ZnSO4 x 7 H2O | 0.18 | g |
CuSO4 x 5 H2O | 0.01 | g |
KAl(SO4)2 x 12 H2O | 0.02 | g |
H3BO3 | 0.01 | g |
Na2MoO4 x 2 H2O | 0.01 | g |
NiCl2 x 6 H2O | 0.03 | g |
Na2SeO3 x 5 H2O | 0.30 | mg |
Na2WO4 x 2 H2O | 0.40 | mg |
Distilled water | 1000.00 | ml |
First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Vitamin solution: | ||
Biotin | 2.00 | mg |
Folic acid | 2.00 | mg |
Pyridoxine-HCl | 10.00 | mg |
Thiamine-HCl | 5.00 | mg |
Riboflavin | 5.00 | mg |
Nicotinic acid | 5.00 | mg |
D-Ca-pantothenate | 5.00 | mg |
Vitamin B12 | 0.10 | mg |
p-Aminobenzoic acid | 5.00 | mg |
Lipoic acid | 5.00 | mg |
Distilled water | 1000.00 | ml |
For DSM 1498 and DSM 22353 adjust pH to 6.5. | ||
For DSM 2373 increase the amount of trypticase to 6.00 g/l. | ||
For DSM 7268, DSM 7466 and DSM 14266 use only one atmosphere overpressure of sterile 80% H2 and 20% CO2 gas mixture.
For DSM 15219, DSM 18860 and DSM 21220 adjust pH to 7.5.
For DSM 15558 supplement medium after autoclaving with 0.50 g/l coenzyme M (2- mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under 100% N2 gas. Adjust pH to 6.5 and use only one atmosphere overpressure of sterile 80% H2 and 20% CO2 gas mixture.
For DSM 16458 supplement medium after autoclaving with 0.50 g/l coenzyme M (2- mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under 100% N2 gas. Adjust pH to 7.5 and use only one atmosphere overpressure of sterile 80% H2 and 20% CO2 gas mixture.
